Before setting a gating strategy, it is vital to know the properties of cells to be analyzed, such as cell size, the relative expression of markers and whether artifacts are likely to have been ...

Differences in how users gate populations within experiments are major sources of variability in flow cytometry data analysis. Incorporating automated image analysis can substantially reduce user bias ...

Subjectivity in flow-cytometry data analysis has long been a challenge. Recent advancements in high-speed camera capabilities have led to the integration of morphological features with traditional ...

The Flow Cytometry shared resource stays at the leading edge of technology. Close ties to industry allow us to offer users early access to new reagents and instrumentation. We help users realize a ...

A group from the Shanghai Jiao Tong University School of Medicine has outlined a protocol for the tissue-resident myeloid populations in mouse tissues using flow cytometry. The protocol builds on ...

Immunophenotyping is the identification and quantitation of heterogeneous populations of cells by multi-parameter flow cytometry using a panel of fluorescently-labelled antibodies that recognize ...

The Cytometry and Cell Sorting Core at Baylor College of Medicine is heavily subsidized by the College and by several NIH-funded Centers. It is an NIH requirement that any publication using equipment ...

Around the same time, Mack Fulwyler, an engineer working at Los Alamos National Laboratory, needed to separate particles, so he drew on existing techniques to create droplets to separate cells from a ...

Flow cytometry is a way to look closely at the features of cells or particles. A sample of blood or tissue goes into a machine called a cytometer. In less than a minute, a computer can analyze ...