This is the second article in this series discussing HPLC oligonucleotide separations. This article will discuss the separation of oligonucleotides and dsDNAs using a wide-pore, polymer based reversed ...

Normal phase HPLC is a valuable technique for the separation of polar and hydrophilic compounds and for isomer separation. Normal phase HPLC requires a polar stationary phase combined with non or ...

The authors describe the development of a stability-indicating reverse-phase high-performance liquid chromatographic (RP?HPLC) method for the quantitative determination of potential genotoxic ...

An interview with Dr. Michael Dong, about his upcoming talk at Pittcon. Why are stability-indicating methods important in pharmaceutical analysis? The stability-indicating assay is perhaps the single ...

Pharmaceuticals need to be assessed for stability to support the assigned shelf life. Therefore, when analysing stability samples obtained from these studies, analytical methods are required which are ...

High performance liquid chromatography, more commonly known by the acronym HPLC, is a method used to identify substances in a mixture. There are several types of HPLC, such as reversed-phase, ...

Learn how to select appropriate method conditions and perform suitable investigative experiments to obtain a set of method parameters which enables the desired separation for mixtures of analytes. In ...

High-performance liquid chromatography is supposed to be complicated, right? Imagine trying to find the correct mobile phase, stationary phase, column length, diameter, particle size, solvent strength ...

High performance liquid chromatography (HPLC) has become an essential technique in pharmaceutical analysis for the separation, identification, and quantification of pharmaceuticals. Due to its high ...

Any analytical method must strike a balance between its intrinsic scientific potential its capacity for robustness and reliability, particularly in regulated industries. In the case of HPLC, this ...