Sucrose concentration in a fermentation broth is measured using high-performance liquid chromatography (HPLC). Chromatogram peak areas are measured for five standard sucrose solutions to calibrate the instrument. Measurements are performed in triplicate with results as shown in the following table. Sucrose concentration (g^-1) Peak area 6.0 55.55,57.01,57.95 12.0 110.66,114.76,113.05 18.0 168.90,169.44,173.55 24.0 233.66,233.89,230.67 30.0 300
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