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Sanger sequencing

YouTubeQuick Biochemistry Basics

Sanger sequencing

This is an animated video on DNA sequencing by Sanger's method. Sanger sequencing method is based the use of dideoxy nucleotides in the DNA polymerizing reaction. The hydroxyl group attached at the 3' end plays a very important role in DNA polymerization reaction during DNA polymerization, There is a reaction between 3'OH group and the ...

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